常用载体测序引物列表

载体名称 正向引物 反向引物
pACT T7 T3
pACT2 GAL4 AD(5’AD) 3'AD/pACT2-R
PACYCDUET-1(MCSII) DuetUP2 T7TER
pAD/pl-DEST CMV-F  
pAD-Track pAD-Track-F
pAS2-1 5’BD M13-26/48
PB42AD PB42ADF PB42ADR
PBAD PBAD-F PBAD-R
pBABE pBABE5’ pBABE3’
PBACPAK8 BAC1 BAC2
pBK-CMV T7/M13-20 T3/M13-26
PBS(SK/KS)/M13- M13F/T7 T3/M13R
pBV220 PBV220F PBV220R
pBI121 需根据酶切位点确认 需根据酶切位点确认
pCAMBIA 1301(1300) 需根据酶切位点确认 需根据酶切位点确认
pCAMBIA 2300 需根据酶切位点确认 需根据酶切位点确认
PCANTB5E S1/M13R S6
pCAT3-enhancer pGL3+  
pcDNA3.0 pEGFP-N5/T7 SP6/BGH
pcDNA3.1 pEGFP-N5/T7 BGH
pcDNA4 T7/pEGFP-N5 BGH
pcDNA6 T7/pEGFP-N5 BGH
pcDNAII T7 SP6
pCE2.1 M13F M13R
pCEP4 pCMV5R/PCEP-F pEGFP-N5/EBV-R
pCF-T M13F M13R
pCI  T7(17Base) pCMV5R
pCI-neo T7(17Base) T3
pCMS-EGFP T7 T3
pCMV-3Tag-4A T3 /pFlag-CMV-F T7
pCMV5 pCMV5F pCMV5R
pCMV5-Flag pCMV5F pCMV5R
pCMV-MYC/NUC pCMV5F pCMV5R
PCMV-HA pCMV5F pCMV5R
pCMV-Sport   SP6/M13R
pCMV-Tag T3 T7
pCMV-TNT T7/SP6 pCMV5R
pCR2.1-TOPO M13F/T7 M13R
pCR3.1 T7 BGH
pCS2 SP6 T7
PDNR-LIB M13F M13R
pDonar M13F M13R
pDONR201 pDONR-F pDONR-R
pDONR221 M13F M13R
pDrive T7 SP6
pDRIveR T7 SP6
pDsRED1-C1 pDsRED-C1-F pECFP-C-3
pDsRed1-N1 pEGFP-N-5 RFP-Nrev
pDsRed-Monomer-N1 pEGFP-N-5 pDsRed1-N
pDsRED2-C1 pDsRED-ex-C1-F pEGFP-N-3’
pDSRED2-N1  pEGFP-N-5 pDsRed1-N
pDSRED-N1 pEGFP-N-5’ PDSRED-N-R
pEASY-BLUNT M13F/T7 M13R
pEASY-E1 T7 T7 Ter
pEASY-T1 M13F/T7 M13R
pEASY-T3 M13F/T7 M13R/SP6
pECFP-C pEGFP-C-5’ pECFP-C-3' 
pECFP-N1 pEGFP-N-5’ pEGFP-N-3’
pEF/myc/ER pEF-F pCDNA3.1R
pEGFP-C pEGFP-C-5’ pEGFP-C-3'
pEGFP-N pEGFP-N-5’ pEGFP-N-3’
pENTR/D-TOPO M13F M13R
pET-*(His) T7 T7 Ter
pET22(a,b,c) T7 T7 Ter
pET28,30,24,49 T7 T7 Ter
pET32(a,b,c) T7/S.tag T7 Ter
pET-42a (-b, -c)(+) Stag T7 Ter
pET50 T7/s.tag T7TER
pET44 T7/s.tag T7 Ter
pEYFP-N1 pEGFP-N-5’ pEGFP-N-3’
PETBlue M13-20 PETBlueDOWN
PETDUET-1(MCSI) PBRrevBam DuetDOMN1
PETDUET-1(MCSII) DuetUP2 T7TER
pEYFP-C1 pEGFP-C-5’ pEGFP-C-3' 
pEYFP-N1 pEGFP-N-5’ pEGFP-N-3’
pFastBac pFastBac-F pFastBac-R
pFastBac HT_A,B,C pFastBac-PH pECFP-C-3
pFastBac1 pFastBac-PH pECFP-C-3
pFastBac Dual (MCSI) pFastBac-PH pECFP-C-3
pFLAG-CMV  CMV30/pFLAG-CMV-F CMV-24/pFLAG-CMV-R
p3xFlag-CMV-7.1 CMV-F/CMV24 CMV30
pGAD424  5’AD 3'AD
pGADGH  5’AD 3'AD
pGADT7-Rec 5’AD/T7 3'AD
pGAPZa-A/B/C a-FACTOR  3'AOX
pGAPZ-A/B/C pGAP forward 3'AOX
pGBKT7 T7 3'BD
pGEM-T(-Easy) M13F/T7 SP6/M13R
pGEX-(*)T pGEX-5’ pGEX-3’
pGL2 pCMV5R PGL3-
Pgl3-BASIC pGL3+ PGL3-
pGL3-Enhancer pGL3+ PGL3-
PinPoint TM PinPoint Primer SP6
pIRES  PIRES-F/T7 T3/PIRES-R
pIRES2-EGFP pIRES2-EGFP.P5’ pIRES2-EGFP.P3’
pLenti6/v5-Dest pEGFP-N-5’  
PLEXA PLEXA-F PLEXA-R
PLNCX PLNCX-F PLNCX-R
pLXSN  pLXSN-F pLXSN-R
Pjc1-tac T7  
pJet.1.21.Blunt T7  
pMAL-c2E MalE primer M13F(-47)
pMAL-C2x P5/PMAL-C2X-F P3/PMAL-C2X-R
pMAL-p2X MalE primer M13F(-47)
PMD18-T M13R(-48) M13F(-47)
PMD19-T M13F(-47) M13R(-48)
pMIR-report M13F  
PPC86 PPC86-F PPC86-R
pPIC9K 5’AOX/a-factor 3’AOX
pPICZa 5'AOX/PPICZa-F 3'AOX
pPROEXHTA M13R(-48)  
pQE30or40 pQE30+ pQE30-
pRECEIVER CMV-F  
pRSET T7 T7TER
PRSFDUET-1(MCSI) DuetUP1 DuetDOWN1
PRSFDUET-1(MCSII) DuetUP2 T7TER
pShuttle-CMV pShuttle-CMV-F pShuttle-CMV-R
PSILENCE-1.0-U6 T7 T3
pSilencer3.1-H1 hygro M13F(-47) 3.0rev
pSILENCEV2.0-U6 T7 2.0rev
pSK01-T M13F M13R
pSK-CMV T7 T3
Psos H1  
PSP64/65 SP6  
pSP72 T7 SP6
pSport1 SP6/M13F(-47) T7/M13R
pSTBlue-1 T7 SP6
pSUPER T7 T3
pT7Blue(R) T7 M13F(-47)
pTA2  M13F/T7 T3/M13R
pT-Adv M13F /T7 M13R
pTARGET TM pTarget-F/T7 arget-R
PTHIOHISA,B,C TRX-FORWARD TRX-REVERSE
pTO-T7 T7 T7TER
pTRC99a-c PQE30+/PTRC99C-F PBV220-/PTRC99C-R
pTriPLEx2 PT5/5'TriplEx2 T7
pTz57r/t M13+ M13-
pTWIN-1 T7 T7TER
pUC18(19)/118(119) M13F(-47) M13R(-48)
pUC57 M13R(-48)/M13R M13F(-47)/M13F
pUCm-T M13F/T7 M13R
pVAX1 T7 BGH
pWSK29 T7/M13F/M13F(-47) T3/M13R/M13R(-48)
pXT7  T7 SP6
pYES2  T7 pYes2.R